The antimicrobial activity was determined in the extracts using agar disc diffusion method. The extracts were filtered using Whatman filter paper No. Like any drugs, the Acapulco extract was also tested for its side effects. The year stamped another important milestone in herbal medicine research and development. It can grow up to 15 feet tall, and has green alternate leaves, with even, pinnate leaflets.

Akapulko has several names from various regions of the country. Am J Clin Pathol. The haphazard use — just to facilitate treatment of a given disorder — enhances other body processes which could adversely promote other body activities. Cassia alata is the plant used in this research in order to further discover if its leaf extract is effective in treating bacteria, specifically those that cause skin infections. This study will be focusing on the Minimum Inhibitory Concentration MIC of the Cassia alata leaf extract that will inhibit growth of the gram-positive bacteria Staphylococcus aureus.

The present study justified the claimed uses of leaves in the traditional system of medicine to treat various infectious disease caused by the microbes.

research paper about akapulko

This study was carried out with an objective to investigate the antibacterial and antifungal potentials of leaves of Akauplko fistula Linn. In the present work, the extracts obtained from Cassia fistula show strong activity against most of the tested bacterial and fungal strains. The hydroalcoholic extracts of Cassia fistula were found to be active on most of the clinically isolated microorganism and fungi, as compare with standard drugs.

The acetone and ethanol extracts showed high activity against nearly all test microorganisms.


The risk from long-term use of such remedies has not however been fully investigated, especially in terms of their potential to cause mutagenicity and resezrch. Additionally, this plant has been investigated to have antibacterial activity against gram positive and gram negative bacteria. Akapulko is known for its dark green compound leaves consisting orange rachis with numbers of leaflets.

Research Instrument The study will use vernier caliper to measure the zone of inhibition. It is then pulverized aka;ulko clean mortar and pestle to fine powder.

The hydroalcoholic extract yields a dark greenish solid residue weighing 5. One of the activities that can be found in traditional plants is the antibacterial activity.

research paper about akapulko

In the study conducted by Dauda H. According to Abubacker et al. In the study they evaluated the antiseptic potentials of Cassia alata- based herbal soap formulated in our laboratory.

These plant materials are either used alone or formulated into local soaps, ointments and akaphlko which are often commercially available. There are various conditions that may affect a disk diffusion test. Antibacterial activity of isolates from Piper longum and Taxus baccata. New aspects of natural products in drug discovery. Which concentration level will give effective inhibition and less inhibition on S. Help Center Find new research papers in: Indian J Agr Sci.

The effectiveness of homemade akapulko ointment in the treatment of fungal infection.

It also serves as herbal medicine for asthma and expectorant for bronchitis Aruh, et. Table 3 Antibacterial activities of hydroalcoholic extracts of leaves of Cassia fistula against fungal test organism. Preparation Using an inoculating loop, obtain an inoculum from the pure culture of Candida albicans and suspend it directly into a small volume of sterile NSS.


The sterile discs will then be soaked in beakers containing 20ml of the prepared Cassia alata-ethanol solution of different concentrations; and prepared commercialized drug-ethanol solution for 12 hours. Open in a separate window. Further studies which aimed at the isolation and structure elucidation of antibacterial active constituents from the plant have been initiated. Alzoreky NS, Nakahara K. With an increase in the antibacterial agent-resistant strains of microorganisms, traditional plants are being explored for their antibacterial and medicinal values Mahuli et al, Antibiotic susceptibility testing by standardized single disc method.

HERDIN – Research Details

A continuing source of novel drug leads. Furthermore, the range of the antibacterial property of the leaf extract will be determined by measuring the diameter of the clear zone of inhibition surrounding the paper disk. It saw the transfer of akapulko lotion as anti-fungal to the private sector for commercialization. New Gyan Offset Printers; In previous studies, Cassia alata has been found to possess excellent wound-healing properties Benjamin and Lamikanra, ; Palanichamy et al.

The powdered sample material will be soaked in ethanol in different concentrations for 48 hours.